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One may wash one’s hands for the sake of a mitzva because such washing is not for the purpose of gaining pleasure. Therefore, Kohanim may wash their hands in preparation for Birkat Kohanim (Rema 613:3, sa 128:6). However, one may not immerse in a mikveh on Tisha Be-Av.7
Upon awakening in the morning, each person must wash his hands from the tips of the fingers to their base, because an evil spirit rests on one’s hands after a night’s sleep, and it can harm the orifices of one’s body. In order to remove this spirit, one must wash each hand three times alternately. After using the bathroom, one should wash his hands again once and recite the berakha of "al netilat yadayim," because the Sages instituted a mitzva to wash one’s hands with a berakha in preparation for Shaĥarit. Even though we usually take care to wash the entire hand, on Tisha Be-Av one should wash only up to the base of one’s fingers, including the knuckles, because technically that is sufficient both for preparing for Shaĥarit and for removing the evil spirit (sa 613:2).8
Le-khatĥila, one should wash his hands before every prayer service throughout the year. Nevertheless, on Tisha Be-Av one should not wash his hands before praying, because doing so is not obligatory. However, if one touched filthy parts of his body and wants to recite sacred words, he should wash his hands, because this washing is for the sake of a mitzva, not in order to derive pleasure (mb 613:5-6, Kaf Ha-ĥayim 613:6).
There is uncertainty regarding the status of one who relieves himself without touching any part of the body that is usually covered, as perhaps he does not need to wash, since he did not touch any filth. In order to avoid this uncertainty, when one relieves oneself on Tisha Be-Av it is best to touch a part of the body that is usually covered and sweaty. This way, all agree that one may wash his hands until the base of one’s fingers, including the knuckles, in order to recite the berakha of Asher Yatzar in a state of cleanliness (sa 613:3, mb ad loc. 4).9

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